RNAi Silencing of Aflatoxin Synthesis
Silencing of Aflatoxin Synthesis Through RNA Interference (RNAi) in Peanut Plants
The overall goal of this project is to use RNA interference (RNAi) to reduce aflatoxin contamination of peanut seeds.
PMIL funds the study of genetic diversity of fungus that produces aflatoxin in crops. Paired with research funded by the NBCRI, the work is could transform peanut plants to stop attached fungus from creating toxin.
For the genetic diversity studies, samples are being analyzed from Ethiopia, Kenya, Malawi, Uganda, Zambia and the USA and fingerprinted using sequences within the aflatoxin synthesis gene cluster at the African scientists in the project, many of whom have visited the NPRL for hands-on training. National Peanut Research Laboratory (NPRL) in Dawson, Georgia.
Three African peanut varieties (CG 7, JL 24 and ICGV 90704) are being transformed at Kenyatta University in Nairobi, Kenya, using RNAi molecular constructs provided by NPRL. Scientists at the NPRL are providing training and backstopping to the African scientists in the project, many of whom have visited the NPRL for hands-on training.
Dr. Renee S. Arias, Research Pathologist
- Dr. Charles Chen
- Dr. Abdi Mohammed Hassen
International Crops Research Institute for the Semi-Arid Tropics
- Dr. Samuel Njoroge
- Dr. Steven Runo
National Crops Resources Research Institute
- Dr. Julius Serumaga
USDA-ARS National Peanut Research Laboratory
- Dr. Phat Dang
- Dr. Marshall Lamb
- Dr. Victor Sobolev
USDA-ARS Genomics and Bioinformatics Research Unit
- Dr. Brian Scheffler